Journal: Stem Cell Research & Therapy

Article Title: SDF-1 and NOTCH signaling in myogenic cell differentiation: the role of miRNA10a, 425, and 5100

doi: 10.1186/s13287-023-03429-x

Figure Lengend Snippet: In vivo myogenic differentiation of control or miR10a, miR425 or miR5100 mimics transfected SC-derived myoblasts or MIPCs subcutaneously transplantated in Matrigel® and analyzed 21 days after transplantation. A Immunolocalization of skeletal muscle myosin in sections of Matrigel® containing differentiating cells; myosin—green, cell nuclei—blue; C Level of myogenesis associated transcripts in cells transplanted within Matrigel®. Three independent analyzes were performed, all reactions were performed in duplicates. Differences were considered statistically significant when p < 0.05 (marked with asterisks, * p < 0.05). Scale: 20 µm

Article Snippet: 5 × 10 5 of control or transfected MIPCs or SC-derived myoblasts were suspended in 0.5 ml of Matrigel® GFR High Concentration (HC) basement membrane matrix (Corning).

Techniques: In Vivo, Control, Transfection, Derivative Assay, Transplantation Assay

Journal: BMC Cancer

Article Title: Distinct roles for the RNA-binding protein Staufen1 in prostate cancer

doi: 10.1186/s12885-021-07844-2

Figure Lengend Snippet: Staufen1 knockdown inhibits Prostate Cancer cell invasion and motility. Motility and Invasion assays were performed following 48 h of Control (CTL) or Staufen1-shRNA (shStau1) expression. a Western blot analysis of Staufen1 expression showing a representative blot of Staufen1 with β-actin as a loading control in PC3 and DU145 cells. Each representative blot is cropped to show an n = 1 for each cell line from their respective full-length blot. Note that the DU145 cells used for Fig. 4 were also used for Fig. experiments and therefore a different representative image from the same blot was selected for each figure. Quantification of n = 4 is represented normalized to CTL. Data are Mean ± SD, One-Sample T-Test, **P < 0.01, ***P < 0.001. b Cells were seeded into transwell chambers containing membranes coated with or without Matrigel and incubated for 72 h and 24 h for PC3 and DU145 cells, respectively. Representative images of cells that passed through the transwell chamber at 40X magnification, scale bar = 20 μm, are displayed. Average number of cells/field of view is quantified for cell motility (no matrigel) and invasion (matrigel) as a percentage relative to CTL. Quantifications are for all data are n = 4. Data are Mean ± SD, Students T-Test, **P < 0.01, ***P < 0.001. Full length blots are presented in Supplemental Fig.

Article Snippet: Infected cells (2.5 × 10 4 ) were seeded in serum free medium in Corning ® BioCoatTM Control Inserts (#354578) or Corning ® GFR Matrigel ® Basement Membrane Matrix Invasion Chambers (#354480) (VWR International, Ontario, Canada) containing growth medium in the bottom chamber.

Techniques: Knockdown, Control, shRNA, Expressing, Western Blot, Incubation

Journal: PLoS ONE

Article Title: Efficient and robust differentiation of endothelial cells from human induced pluripotent stem cells via lineage control with VEGF and cyclic AMP

doi: 10.1371/journal.pone.0173271

Figure Lengend Snippet: Immunofluorescent staining for (a) CD31 (green) and (b) VE-Cadherin (red) in human iPS cell-derived endothelial cells. Nuclei were visualized with DAPI (blue). (c-g) Relative mRNA log 0 ratio in endothelial cells at differentiation day 9 (EC (D9)) and undifferentiated human iPS cell (iPSC) compared with human umbilical vein endothelial cell (HUVEC). CD31 (c), VE-Cadherin (d), eNOS (e), CD34 (f), and CD133 (g). (h) Tube formation assay. Human iPS cell-derived endothelial cells were recultured on Matrigel Basement Membrane Matrix GFR coated dish. (i) Immunofluorescent stained of CD31 for recultured cells on Matrigel. (j,k) Acetyl-LDL incorporation assay. Endothelial cells were incubated with acetylated LDL labeled with 1,1’-dioctadecyl-3,3,3’,3’-tetramethylindo-carbocyanine perchlorate (DiI-Ac-LDL). Bright-field (j) and fluorescent (k) images. Scale bars: 50 μm in (a), (b) and (i), 100 μm in (h), 200 μm in (j) and (k).

Article Snippet: Briefly, differentiated ECs (7 x 10 4 ) at d13 were plated on a 24-well plate (Thermo Fisher Scientific) coated with 300 μl Matrigel Basement Membrane Matrix GFR (BD Biosciences), and cultured for 24 hrs.

Techniques: Staining, Derivative Assay, Tube Formation Assay, Incubation, Labeling